HCS (High Content Screening)

We are pleased to announce that Soft Flow Hungary Ltd. recently bought a microscope system suitable for HCA (High Content Analysis) studies. Currently (to our knowledge) in addition to our Olympus scan^R platform, there are only two (closed system) HCS units operating in the country.

HCS combines the instruments and methods of molecular and cell biology with automated, high- resolution, light and/or fluorescence microscopy. By the application of this method, a series of pictures can be taken of a great number of cells and organelles, similarly to large statistical populations in the case of flow cytometry. By using diverse quantitative morphological/morphometric methods on the recorded images, and/or by the multiple analysis of the fluorescence images, we are able to create Flow Cytometry Standard (FCS) data files, which are commonly used in flow cytometry, and can be assessed by similar methods as results obtained by flow cytometers. An important component of the method is the image analysis software, which produces digitized data files of the images. The data can be evaluated, analyzed by most flow cytometry analysis software (e.g. our own CellCap software). The outstanding advantage of the HCA method over flow cytometry is that each individual measuring point can be traced back to the examined cell/organelles, from which the data originated.

HSC measurements are typically carried out on cell culture plates, on fixed samples, but the device is suitable for the examination of slides as well. It has an extremely wide range of applications, (e.g. morphology, surface marker analysis, co-localization, cell cycle analysis, internalization, translocation, apoptosis, etc.).

We believe that the use of HCS offers good publishing opportunities in the field of immunology research (as well)!

We are looking for partner universities, research institutes to work together in two areas of HCA application:

  • The services we provide: we measure and evaluate samples of your experiments that you send to us in cell culture dishes/carriers or on slides. We gladly collaborate in designing the experiments.
  • Co-operation in assay development: we are seeking partners for the development of procedures applicable in pharmaceutical research and drug development and for practical applications. We share the revenues obtained from applications.

Application fields

Cell counting, Gene expression, Intracellular transport, Translocation, Cell proliferation, Bacterial infection assay, Cell cycle analysis, Protein localisation and co-localisation, Live cell assays, Multi-colour assays, Rare event analysis, Automated FISH analysis, Fluorescence analysis in tissue sections, Cell array screens, Micronuclei and comet assays, Apoptosis, Calcium Flux, Cell Differentiation, Cell Migration, Cell Shape Changes, Cytoskeletal Rearrangement, Cytotoxicity, Infection, Lipid Droplet Analysis, Protein Expression, Receptor Activation, Reporter Gene, Signaling Pathway Analysis, Transcription Factors, etc.

Recommended literature:

  • Joseph M. Zock (2009) Applications of High Content Screening in Life Science Research (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2841426/)
  • William Buchser, et al.. (2012) Assay Development Guidelines for Image-Based High Content Screening, High Content Analysis and High Content Imaging. (http://www.ncbi.nlm.nih.gov/books/NBK100913/)