Repro-Watch

Progesterone (P4) ELISA kit

 
General information

P4 is an important female sex hormone, and is responsible for reproductive-related activities.

In mammals, P4 also directs pregnancy support physiology including changes in carbohydrate, protein and lipid metabolism, thermoregulation, sodium reabsorption in renal tubules and the reduction of alveolar and atrial carbon dioxide partial pressures.

In mammals, progesterone is secreted in large amounts mainly by the corpus luteum and it can be created/conversed by the extracellular conversion of cholesterol, cholestryl esters, adrenal steroids, prenenolone and pregnenolone sulfate. Small quantities are also secreted directly from the adrenal glands.


The wells of the microtiter plate are coated with Streptavidin, in which users should pipette the standards or samples and the progesterone-horseradish peroxidase (HRP) conjugate.

These components compete for the antigen-binding places of the P4-specific biotinylated monoclonal antibody. Contemporarily that antibody can be intensely bounded on the surface of the wells. All the unbound components can be eliminated with the following wash procedures. The colourless substrate goes blue in proportion with the quantity of bound P4-HRP conjugate. The stop solution (1N H2SO4) stops the reaction and it changes that colour from blue to yellow. Its intensity can be measured with microplate-reader (at 450 nm). The measured absorbance is in inverse proportion to the concentration of P4.

 

Comments are closed.